Publications

2025

Z. Zhang, T.C. Todeschini, Y. Wu, R. Kogay, A. Naji, J. Cardenas Rodriguez, R. Mondi, D. Kaganovich, D.W. Taylor, J.P.K. Bravo, M. Teplova, T. Amen, E. Koonin, D.J. Patel, F.L. Nobrega. (2025) Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites Cell. 10.1016/j.cell.2025.07.002

Rodrigo Fregoso Ocampo, Jack PK Bravo, Tyler L Dangerfield, Isabel Nocedal, Samatar A Jirde, Lisa M Alexander, Nicole C Thomas, Anjali Das, Sarah Nielson, Kenneth A Johnson, Christopher T Brown, Cristina N Butterfield, Daniela SA Goltsman, David W Taylor (2025) DNA targeting by compact Cas9d and its resurrected ancestor. Nature Communications. 10.1038/s41467-024-55573-4

2024

Jack P. K. Bravo^†. (2024) Anti-plasmid immunity: a key to pathogen success? Future Microbiology. 10.1080/17460913.2024.2389720. ^†Corresponding author.

J. P. K. Bravo^†, Delisa A. Ramos, Rodrigo Fregoso Ocampo, Caiden Ingram & David W. Taylor. (2024) Plasmid targeting and destruction by the DdmDE bacterial defence system. Nature. 10.1038/s41586-024-07515-9. ^†Corresponding author.

Dmitrii Degtev*, Jack P. K. Bravo*, Aikaterini Emmanouilidi, Aleksandar Zdravković, Oi Kuan Choong, Julia Liz Touza, Niklas Selfjord, Isabel Weisheit, Margherita Francescatto, Pinar Akcakaya, Michelle Porritt, Marcello Maresca, David Taylor, Grzegorz Sienski. (2024) Engineered PsCas9 enables therapeutic genome editing in mouse liver with lipid nanoparticles. Nature Communications. 10.1038/s41467-024-53418-8

Grace N Hibshman, J. P. K. Bravo^†, Matthew Hooper, Hongshan Zhang, Tyler L Dangerfield, Ilya J Finkelstein, Kenneth A Johnson, David W Taylor^†. (2024) Unraveling the mechanisms of PAMless DNA interrogation by SpRY Cas9. Nature Communications. 10.1038/s41467-024-47830-3 ^†Co-corresponding author.

Schwartz, E. A.*, Bravo, J. P. K.*, et al. (2024) Assembly of multi-subunit fusion proteins into the RNA-targeting type III-D CRISPR-Cas effector complex. Nature Communications. 10.1038/s41467-024-47506-y

Kamatar, A., Bravo J.P.K et al. (2024) Lipid droplets as substrates for protein phase separation. Biophysical Journal. 10.1016/j.bpj.2024.03.015

Jurre Steens*, Jack P.K. Bravo*, Carl Raymund P Salazar*, Caglar Yildiz, Afonso M Amieiro, Stephan Kostlbacher, Stijn HP Prinsen, Constantinos Patinios, Andreas Bardis, Arjan Barendrecht, Richard A Scheltema, Thijs JG Ettema, John van der Oost, David W Taylor, Raymond Staals. (2024) Type III-B CRISPR-Cas cascade of proteolytic cleavages. Science. 10.1126/science.adk0378

2023

Bravo, J. P. K., et al. (2023) RNA targeting unleashes indiscriminate nuclease activity of CRISPR–Cas12a2. Nature. 10.1038/s41586-022-05560-w

O’Brien, R. E., Bravo, J. P. K., et al. (2023) Structural snapshots of R-loop formation by a type I-C CRISPR-Cascade. Molecular Cell. j.molcel.2023.01.024

Yelland, J. N. Bravo, J. P. K., et al. (2023). A single 2’-O-methylation of ribosomal RNA gates assembly of a functional ribosome. Nature Structural and Molecular Biology. 10.1038/s41594-022-00891-8

2022

Bravo, J. P. K.^†, et al. (2022). Constructing the next-generation of CRISPR-Cas tools from structural blueprints. Current Opinion in Biotechnology. 10.1016/j.copbio.2022.102839.^{. †}Corresponding author.

Bravo, J. P. K., et al. (2022). Structural basis for broad anti-phage immunity by DISARM. Nature Communications. 10.1038/s41467-022-30673-1

Schwartz, E. A.*, McBridge, T. M.*, Bravo, J. P. K.*, et al. (2022). Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. Nature Communications. 10.1038/s41467-022-30402-8.

Bravo, J. P. K., et al. (2022). Structural basis for mismatch surveillance by CRISPR-Cas9. Nature. 10.1038/s41586-022-04470-1

2021

Steens, J.A., Zhu, Y., Taylor, D.W., Bravo, J.P.K., et al. (2021) SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation. Nature Communications. 10.1038/s41467-021-25337-5

Zhou, Y.*, Bravo, J.P.K.*, Taylor, H.N.*, et al. (2021). Structure of a type IV CRISPR-Cas ribonucleoprotein complex. iScience. 10.1016/j.isci.2021.102201

Bravo, J.P.K., et al. (2021). Remdesivir is a delayed translocation inhibitor of SARS CoV-2 replication. Molecular Cell. 10.1016/j.molcel.2021.01.035

Geiger, F., Papa, G., Arter, W.E., Acker, J., Saar, K.L., Qi, R., Bravo, J.P.K., et al. (2021). Liquid-liquid phase separation underpins the formation of replication factories in rotaviruses. EMBO Journal. 10.15252/embj.2021107711

Bravo, J.P.K., et al. (2021). Structural basis of rotavirus RNA chaperone displacement and RNA annealing. PNAS. 10.1073/pnas.2100198118

2020

O’Brien, R.E., Santos, I. C., Wrapp, D., Bravo, J. P. K., et al. (2020). Structural basis for assembly of non-canonical small subunits into type I-C Cascade. Nature Communications. 10.1038/s41467-020-19785-8.

2018

Bravo, J. P. K., et al. (2018) Stability of local secondary structure determines selectivity of viral RNA chaperones. Nucleic Acids Research, 10.1101/293191.